Fig. 4.
PRV-1 is N-glycosylated.
Total cell extracts were prepared from granulocytes from patient EM. The extracts were either left untreated or subjected to digestion with 2.4 IU N-glycosidase at 37°C for 21 hours. Proteins were analyzed by Western blot using a polyclonal antibody generated against a PRV-1 peptide (amino acids 13-25 of the mature protein).
