PRV-1 protein expression in the peripheral blood and bone marrow.

(A) Peripheral blood from a healthy control (UPN 686, dotted line) and 2 patients with polycythemia vera (UPN 651, thin solid line, and UPN 419, thick solid line) was stained with the lineage-specific antibodies CD13, CD14, CD19, CD45 as well as an antibody against PRV-1. Peripheral blood granulocytes (i), monocytes (ii), and B cells (iii) were identified by their surface markers and analyzed for PRV-1 expression. Panel A-iv depicts a Northern blot of RNA from purified granulocytes of the same individuals hybridized to the PRV-1 cDNA (top) or to β-actin (bottom). (B) Bone marrow aspirates of a healthy bone marrow donor (UPN 687, dotted line) and 2 patients with polycythemia vera (UPN 457, thick solid line, and UPN 415, thin solid line) were stained with the lineage-specific antibodies CD3, CD13, CD14, CD19, CD45 as well as an antibody against PRV-1. Bone marrow granulocytes (i), monocytes (ii), B cells (iii), and T cells (iv) were identified by their surface markers and analyzed for PRV-1 expression.