Fig. 3.
![Fig. 3. ATRA-induced conformational changes in wild-type and I410T mutant PML/RARα fusion proteins but not in R276W mutant. / Limited trypsin digestion analysis of wild-type and mutant PML/RARα proteins. In vitro [35S]-methionine synthesized PML/RARα proteins were incubated without (−) or with (+) 1 μM ATRA, and were subsequently treated with increasing trypsin concentrations (0-25 μg/mL). Digestion products were analyzed by denaturing electrophoresis. The arrows indicate the intact PML/RARα proteins. Lower and upper asterisks indicate resistant fragments at 32 kDa and 37 kDa, respectively.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/7/10.1182_blood-2002-02-0614/3/h81923183003.jpeg?Expires=1735594079&Signature=0R65Ax-XJn4p3A4rl7oUhRRWqBo8qX2d67hNU6O-2XDoKmFCenKAgdGWq7C6MdFCZN1eN5qHMrgjUfKDTvGk5G2-KEAyHLHQ3~c0o274Y-drhABuTbifEPjh~uSePB6r4Ab8pF~k402tsGvGnK8-pscAqU-phQu57ad1kwQBqU9HXb400ZNVN8jK1Dl71G3dxCK8LPb99qqbLxEIzoh~4qPs-sIxlLrRxQ9OyEBjuddC6urwuM4ZEi1UF43A5KONL4kmVqJy5kuyx0KZ~c9DDRItzUFsK-c~V5c1Sn9t9kbrIkSZXmdBDjhhqSLwvNAWFjn14rBfKOTulHdvrVw6JA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
ATRA-induced conformational changes in wild-type and I410T mutant PML/RARα fusion proteins but not in R276W mutant.
Limited trypsin digestion analysis of wild-type and mutant PML/RARα proteins. In vitro [35S]-methionine synthesized PML/RARα proteins were incubated without (−) or with (+) 1 μM ATRA, and were subsequently treated with increasing trypsin concentrations (0-25 μg/mL). Digestion products were analyzed by denaturing electrophoresis. The arrows indicate the intact PML/RARα proteins. Lower and upper asterisks indicate resistant fragments at 32 kDa and 37 kDa, respectively.
