Fig. 2.
Expression of mutant Rac2D57N, Rac2D57NΔCT, Rac2D57NΔM, and wild-type Rac2 proteins in transduced NIH3T3 and BM-derived myeloid cells.
Equal amounts of total protein from cell extracts from each sample was subjected to SDS-PAGE, blotted onto polyvinylidenefluoride membrane, stained with Ponceau-S (Sigma), and probed with either anti-Flag (M2) or anti-Rac antibodies. Lanes 1-5, NIH3T3 cells; lanes 6-9, bone marrow cells.
