Fig. 4.
Fig. 4. Effect of expression of mutant Rac proteins on neutrophil differentiation. / Wright-Giemsa staining of retrovirus-transduced mononuclear BM cells after in vitro differentiation: (A) EGFP alone, (B-D) Rac2D57N, (E) Rac2D57NΔCT, or (F) Rac2D57NΔM. Wright-Giemsa stained, magnification × 1000. Note the abnormal cell size, multiple nuclei (arrows in B-D), hypersegmented nuclei (arrowhead in B), intense granules and irregular granulation (black arrowhead in D), and asynchronous nuclear and cytoplasmic maturation (B-D) in Rac2D57N-expressing neutrophils. Also note the marked reduction in the number of mature neutrophils present in Rac2D57N-expressing cells. The white arrow in panel D indicates the cell processes extending in a “streamer” morphology predominantly found in Rac2D57N-expressing cells under the experimental conditions. These photomicrographs are representative of the results obtained from 3 independent experiments.

Effect of expression of mutant Rac proteins on neutrophil differentiation.

Wright-Giemsa staining of retrovirus-transduced mononuclear BM cells after in vitro differentiation: (A) EGFP alone, (B-D) Rac2D57N, (E) Rac2D57NΔCT, or (F) Rac2D57NΔM. Wright-Giemsa stained, magnification × 1000. Note the abnormal cell size, multiple nuclei (arrows in B-D), hypersegmented nuclei (arrowhead in B), intense granules and irregular granulation (black arrowhead in D), and asynchronous nuclear and cytoplasmic maturation (B-D) in Rac2D57N-expressing neutrophils. Also note the marked reduction in the number of mature neutrophils present in Rac2D57N-expressing cells. The white arrow in panel D indicates the cell processes extending in a “streamer” morphology predominantly found in Rac2D57N-expressing cells under the experimental conditions. These photomicrographs are representative of the results obtained from 3 independent experiments.

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