Expression of lineage markers on transduced and differentiated BM cells.

After in vitro culture and differentiation for 14 days, BM cells from the respective cultures were stained with PE-conjugated isotype control, antimouse c-Kit, Sca-1, Gr-1, or Mac-1 antibodies. Live cells were gated on EGFP, and expression of the indicated antigens on the EGFP⁺ cells was analyzed. Percentages of cells in M1 gate are indicated. Similar results were obtained in 3 independent experiments.