Immunofluorescence photomicrographs show the localization of Rac proteins in transduced NIH3T3 cells by epifluorescence and confocal microscopy.

Panel A shows vector control; panels B and G, wt Rac2; panel C, Rac2^D57NΔCT; panel D, Rac2^D57NΔM; panels E and H, Rac2^ΔCT; and panels F and I, Rac2^ΔMproteins, which were tagged with a Flag epitope at the N-terminus, using the mouse anti-Flag M2 antibody followed by TRITC-conjugated anti–mouse IgG. (A-F) Epifluorescence images, magnification × 1000; (G-I) immunofluorescence images recorded with a confocal microscope, magnification × 600. Arrowheads indicate the proteins localized to the plasma membrane.