Calcium mobilization.

(A) CCL4-induced calcium mobilization of B-cell subsets sorted from bone marrow. Sorted B cells were loaded with Indo-1amand stimulated at the indicated time points (arrows) with the 1000 ng/mL CCL4. The results are representative of 3 experiments using primary B cells from bone marrow. (B) Inhibition of CXCL12-induced calcium mobilization by CCL4. Pre-B cells were sequentially stimulated with 100 ng/mL CXCL12 first and then restimulated with 1000 ng/mL CCL4 (i) or first with CCL4 and then restimulated with CXCL12 (ii). The results are representative of 2 independent experiments.