Cbfβ is expressed in c-kit^hi cells in AGM and fetal liver embryos.

Cells were isolated from 11.5-dpc AGM (A) and fetal liver (B) ofCbfb^+/+ (+/+), Cbfb^+/GFP(+/GFP), and Cbfb^GFP/GFP(GFP/GFP) embryos and stained with c-kit PE. Representative contour plots show the distribution of cells with respect to GFP and c-kit fluorescence. (C) Cells were isolated from fetal liver of 14.5 dpcCbfb^+/GFPembryos and sorted into c-kit⁺/GFP⁻ and c-kit⁺/GFP⁺ populations by FACS. Progenitors in each of these populations (sorted and unsorted) were assessed by methylcellulose colony assay. The data from the methylcellulose colony assays are shown in Table 2.