Electrophoretic and immunostaining of the proteins of the band 3 complex and of the Rh complex.

Erythrocyte ghost membranes were separated on 8% or 10% Laemmli gels. Loading: C1, C2, C3, C4, controls 1-4; M, mother; P, proband. Immunoblotting used polyclonal antibodies against protein 4.2, C-terminal region of CD47 and Rh polypeptides, and monoclonal antibodies: BRIC 170 (band 3), BRIC 163 (GPA), BRIC 125 (N-terminal region of CD47), LA1818 (RhAG), BS56 (LW), and R1.3 (GPB).