American Society of Hematology

Fig. 6.

$Fig. 6. Analyses of bone marrow hematopoiesis in transgenic-EpoR rescued mice. / (A) FACS analysis of the mononucleated cells in Rescued-A mice. PI-negative cells were gated, and fluorescence intensities were analyzed. (B) RT-PCR analysis of sorted bone marrow cells from Tg-A and Rescued-A mice. GFP-positive (+), GFP-negative (−), and total (T) fractions were sorted, and RNA was extracted. Endogenous EpoR, transgenic EpoR, and GATA-1 transcripts were detected by RT-PCR. Negative (nc) and positive (pc) controls were loaded in lanes 7 and 8, respectively.$

Analyses of bone marrow hematopoiesis in transgenic-EpoR rescued mice.

(A) FACS analysis of the mononucleated cells in Rescued-A mice. PI-negative cells were gated, and fluorescence intensities were analyzed. (B) RT-PCR analysis of sorted bone marrow cells from Tg-A and Rescued-A mice. GFP-positive (+), GFP-negative (−), and total (T) fractions were sorted, and RNA was extracted. Endogenous EpoR, transgenic EpoR, and GATA-1 transcripts were detected by RT-PCR. Negative (nc) and positive (pc) controls were loaded in lanes 7 and 8, respectively.

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