CpG-ODN therapy increases the number of naive (CD44^low) and memory (CD44^high) T cells in normal and thymectomized mice.

Thymectomized (white bars) and normal (gray bars) C57BL/6 mice (3 animals per group) were treated with 9 injections of the CpG-ODN (hatched bars) or PBS (solid bars) as described in “Materials and methods.” The percentages of the various subsets of T cells on draining lymph nodes or spleens were determined by 2-color flow cytometry. Results represent the total cell yield calculated by multiplying the proportion of cells from each subset by the number of cells from CpG-ODN mice divided by the PBS control for each treatment group. The error bars represent the standard error of the means obtained from 3 separate experiments.