Fig. 1.
Fig. 1. Segregation of BrdU-labeled and unlabeled DCs. / DCs were isolated, stained for DC surface markers and intracellular BrdU, and analyzed by flow cytometry gating on the CD11c+ MHC class IIhi population. Results are typical of those after 2 days of continuous BrdU administration for spleen DCs and 10 days for LN DCs. The continuous line indicates the BrdU-fluorescence staining after BrdU administration, and the broken line indicates the background staining of DCs from mice not administered BrdU but with identical, side-by-side, isolation and staining protocol.

Segregation of BrdU-labeled and unlabeled DCs.

DCs were isolated, stained for DC surface markers and intracellular BrdU, and analyzed by flow cytometry gating on the CD11c+ MHC class IIhi population. Results are typical of those after 2 days of continuous BrdU administration for spleen DCs and 10 days for LN DCs. The continuous line indicates the BrdU-fluorescence staining after BrdU administration, and the broken line indicates the background staining of DCs from mice not administered BrdU but with identical, side-by-side, isolation and staining protocol.

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