BrdU-labeling kinetics of subtypes of thymic DCs.

Conditions were similar to those for Figure 2, except that the thymic DCs were subdivided based on surface marker differences. Upper graph: the 60% staining brightest with anti-CD8α (CD8α⁺) versus the 30% showing the lowest staining (CD8α⁻). Lower graph: the 50% showing brightest staining with anti-MHC class II(hi) versus the 50% showing less bright staining (lo). In MHC class II staining, the distribution was continuous and the division arbitrary; other cutoff points between low and high MHC class II expression gave similar results. Results are pooled from 4 separate kinetic-labeling experiments.