Fig. 6.
Tracking the movement of DCs from skin to LN by labeling the skin with a fluorescent dye.
The ear skin was painted with TRITC to mark DCs and DC precursors. Identification of skin-derived TRITC-positive gated DCs in the draining auricular LN is shown (A). DC recovery from the auricular LN of control mice before ear painting was 15 × 104 DCs per mouse, a value lower than but not statistically significant different from the recovery from ear-painted mice. The expression of DEC-205 and Langerin by the gated TRITC-positive DC is shown (B); the broken line gives the background, omitting only the specific mAb. Data represent one of 3 experiments giving similar results.
