Interaction of FANCD2 and RAD51 during S phase.

PD20 (FA-D2) cells were stably transfected with the cDNA encoding the amino terminal HA-epitope–tagged version of FANCD2. (A) Cells were examined for MMC sensitivity. (B) Cells were synchronized by the double-thymidine–block method, and synchrony was confirmed by FACS analysis (data not shown). Double-label immunofluorescence (IF) was performed with a murine monoclonal antibody to HA (HA.11; green) and a rabbit polyclonal antibody to RAD51 (Ab-1; red). Where green and red signals overlap, a yellow pattern is seen, indicating colocalization of FANCD2 and RAD51. Original magnification, × 600.