Adhesion of human RSM cells and chemoinvasion of RH28 and RH30 cells.

(A) Adhesion of human RMS cells to fibronectin or laminin; (i) adhesion to laminin, (ii) adhesion to fibronectin, and (iii) adhesion to BSA. Cells not stimulated with SDF-1 are shown as 100% (0), cells stimulated with SDF-1β are RH30 (1), RH28 (2), CW9019 (3), SMS-CTR (4), and RD (5). Data from 4 separate experiments are pooled together. *Indicates P < .01. (B) Chemoinvasion of RH28 and RH30 cells across the Matrigel barrier. Cells invading the Matrigel were counted on the undersides of the filters as described in “Materials and methods.” Results are expressed as a chemoinvasion index. Left part of the panel shows the number of cells crossing the Matrigel in the absence of an SDF-1 gradient (−/−), and middle panel the number crossing in the presence of an SDF-1 gradient (−/+). Right part of panel shows reduced chemoinvasion toward an SDF-1 gradient after preincubation of cells with the MMP inhibitoro-phenanthroline (phen/+). Data from 3 separate experiments are pooled together and means ± SD are shown.