T140 inhibits the SDF-1–directed metastatic behavior of RMS cells.

(A) Effect of T140 on adhesion of RH30 (red bars) and CW9019 (blue bars) cells to HUVECs. Upper panel, relative number of the cells; lower panel, calcein-am–labeled RH30 cells present on HUVECs, as detected by immunofluorescence microscopy. (B) Effect of T140 on chemotaxis of RH30 (red bars) and RH28 (blue bars) to the cultured bone marrow stromal cells. Upper panel, relative number of cells; lower panel, RH30 cells present on the lower side of pore membrane stained by hematoxylin-eosin. The experiments were repeated 4 times with similar results. Original magnifications: panel A, × 100; panel B, × 400.