![Fig. 6. Effect of the expression of Cdc42N17 on the activation of PLCβ2. / (A) fMLFK-mediated IP3 formation. IP3 levels in differentiated Cdc42N17-expressing cells (black bars) and uninduced cells (gray bars) were determined at time intervals after stimulation with fMLFK (1 μM) using an inositol-1,4,5-trisphosphate [3H] radioreceptor assay kit. Error bars represent the standard deviation of 3 independent experiments performed in duplicate. (B, C) Kinetics of fMLFK-mediated intracellular calcium mobilization. Cells were loaded with Fura-2, and fMLFK-mediated calcium increase was assayed in the absence of extracellular calcium. Mean responses ± SE (n = 7) in differentiated Cdc42N17- (B) or Cdc42V12- (C) expressing cells grown in the absence (triangles) or in the presence (circles) of doxycycline are presented. (D) Effect of ionomycine on superoxide production in Cdc42N17-expressing HL-60 cells. Cells grown and differentiated in the absence of doxycycline were treated or not for 2 minutes with 1 μM ionomycine at 37°C before stimulation with fMLFK. Data are representative of 2 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/5/10.1182_blood-2001-12-0193/3/h81723025006.jpeg?Expires=1735843063&Signature=RM4VF-ki9NNM64xTHsCVAO937gXtR-Uo7hXpzdpRjdR6wFwYOqf8nLG2EOpkDrZggPcAJ5A~hTT1TrrdAep8r3SuWOCFqT-L-miB-B3aIiqwhRZ4bZ8MgCwRgtv5HvGbippJFLMwDEyUb0KRWBNccLFT-og~-4U3~2VGwfg78MkDds4Nooto-1E8Vk1zLnpXhdA46uR1NoHiifp5trbWp31xLsRhc3x6WsmKiOlF2Gf5a1t-pWI8UNfZmNB0se-t9hHmdsR1BVrxFoolKQXFRPmDEy5Kp9RhYeynC~HlXG-cWbLbmBLyTzSLFQVZchOiAbjgzGZiVpu21pAGreo8Ww__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of the expression of Cdc42N17 on the activation of PLCβ2.
(A) fMLFK-mediated IP3 formation. IP3 levels in differentiated Cdc42N17-expressing cells (black bars) and uninduced cells (gray bars) were determined at time intervals after stimulation with fMLFK (1 μM) using an inositol-1,4,5-trisphosphate [3H] radioreceptor assay kit. Error bars represent the standard deviation of 3 independent experiments performed in duplicate. (B, C) Kinetics of fMLFK-mediated intracellular calcium mobilization. Cells were loaded with Fura-2, and fMLFK-mediated calcium increase was assayed in the absence of extracellular calcium. Mean responses ± SE (n = 7) in differentiated Cdc42N17- (B) or Cdc42V12- (C) expressing cells grown in the absence (triangles) or in the presence (circles) of doxycycline are presented. (D) Effect of ionomycine on superoxide production in Cdc42N17-expressing HL-60 cells. Cells grown and differentiated in the absence of doxycycline were treated or not for 2 minutes with 1 μM ionomycine at 37°C before stimulation with fMLFK. Data are representative of 2 independent experiments.
