American Society of Hematology

Fig. 8.

$Fig. 8. The expression of Cdc42N17 inhibits Ras activation. / (A) Differentiated induced or uninduced cells were stimulated for indicated time with 1 μM fMLFK or 1 μg/mL PMA. Cells from withdrawn aliquots were lysed, and Ras-GTP pull-down was performed using a GST-fusion of the Raf1 Ras-binding domain. The isolated Ras was detected after SDS-PAGE by Western blot analysis using an anti-Ras antibody. (B) A fraction of an unstimulated cell lysate was probed with an anti-Ras antibody. The figure is representative of 3 independent experiments.$

The expression of Cdc42N17 inhibits Ras activation.

(A) Differentiated induced or uninduced cells were stimulated for indicated time with 1 μM fMLFK or 1 μg/mL PMA. Cells from withdrawn aliquots were lysed, and Ras-GTP pull-down was performed using a GST-fusion of the Raf1 Ras-binding domain. The isolated Ras was detected after SDS-PAGE by Western blot analysis using an anti-Ras antibody. (B) A fraction of an unstimulated cell lysate was probed with an anti-Ras antibody. The figure is representative of 3 independent experiments.

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