American Society of Hematology

Fig. 9.

$Fig. 9. Expression of Cdc42N17 interferes with Rac2 activation. / (A) Differentiated induced or uninduced cells were stimulated with 1 μM fMLFK or 1 μg/mL PMA. Aliquots were withdrawn at indicated times. Cells were lysed, and Rac-GTP pull-down was performed using a GST-fusion of the PAK1 Rac-binding domain. The isolated Rac2 was detected after SDS-PAGE by Western blot analysis using an anti-Rac2 antibody. (B) A fraction of an unstimulated cell lysate was probed with an anti-Rac2 antibody. Results are representative of 4 different experiments.$

Expression of Cdc42N17 interferes with Rac2 activation.

(A) Differentiated induced or uninduced cells were stimulated with 1 μM fMLFK or 1 μg/mL PMA. Aliquots were withdrawn at indicated times. Cells were lysed, and Rac-GTP pull-down was performed using a GST-fusion of the PAK1 Rac-binding domain. The isolated Rac2 was detected after SDS-PAGE by Western blot analysis using an anti-Rac2 antibody. (B) A fraction of an unstimulated cell lysate was probed with an anti-Rac2 antibody. Results are representative of 4 different experiments.

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