American Society of Hematology

Fig. 10.

$Fig. 10. Expression of Cdc42N17 inhibits the agonist-induced translocation of Ras-GRF to the membrane fraction. / Cells were differentiated with Bt2cAMP in the presence or absence of doxycycline. Aliquots were treated with 1 μM fMLFK for the indicated times and lysed. After subcellular fractionation, membrane fractions were submitted to SDS-PAGE. The amount of Ras-GRF at the membrane was determined by Western blotting with an anti–Ras-GRF antibody. The figure is representative of 3 different experiments.$

Expression of Cdc42N17 inhibits the agonist-induced translocation of Ras-GRF to the membrane fraction.

Cells were differentiated with Bt₂cAMP in the presence or absence of doxycycline. Aliquots were treated with 1 μM fMLFK for the indicated times and lysed. After subcellular fractionation, membrane fractions were submitted to SDS-PAGE. The amount of Ras-GRF at the membrane was determined by Western blotting with an anti–Ras-GRF antibody. The figure is representative of 3 different experiments.

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