HA-FANCE interacts with FANCA, FANCC, and FANCG in vivo.

Whole-cell extracts were generated from HeLa cells stably transfected with HA-FANCE. The same amount of protein from the extract (2 mg) was used for immunoprecipitation with affinity-purified anti-FANCA, anti-FANCC, anti-FANCG, anti-FANCD2 antibody, or preimmune serum, as indicated. Immune complexes were resolved by SDS-PAGE, transferred to nitrocellulose, and immunoblotted with anti-FANCA, anti-FANCC, anti-FANCG, anti-FANCD2, or anti-HA antibody.