Fig. 2.
The P2X1-mediated ERK2 phosphorylation depends on Ca++ and PKC.
Western blot analyses of α,β-meATP–induced ERK2 activation in apyrase-treated washed platelets using the same antibodies as in Figure1. (A) α,β-meATP–induced ERK2 activation was analyzed after 2 minutes with or without 2 minutes of preincubation with the MEK inhibitor U-0126 (1 μM) or the PKC inhibitor GF109203-X (10 μM), as indicated. (B) Dose response to α,β-meATP in the presence of 5 mM EGTA. (C) Platelets were stimulated for 2 minutes with 5 μM ADP.
