Fig. 3.
Selective P2X1-mediated ERK2 phosphorylation in transfected HEK293 cells.
Western blot analyses of ERK1/2 phosphorylation in apyrase-treated HEK293 cells, stably expressing normal P2X1(P2X1wt) or its dominant-negative P2X1delL mutant. ERK1/2 activation was induced with 100 μM α,β-meATP in the presence of 2 mM CaCl2.