Fig. 4.
Role of P2X1-mediated ERK2 activation in platelet ATP secretion and aggregation induced by low concentrations of collagen.
Parallel recordings of light transmission (%) and ATP secretion (μM) in hirudinized PRP during platelet stimulation with 0.5 μg/mL collagen alone (control), in the presence of 0.5 μM α,β-meATP, after a 2-minute preincubation with 1 μM U0126, or in the presence of 10 mM EGTA, as indicated. Arrows represent the time of application of collagen. Dashed arrows indicate the moment of initial detection of ATP during platelet aggregation. Percentages of light transmission, the concentrations of ATP released, and the time bar are included.
