Entire strategy for the identification and characterization of DC-associated genes.

DC denotes an equal mixture of 3 different DC subsets, including CD1a⁺, CD11c⁻, and CD14⁺ DCs. BMT denotes the mixture of B cells, monocytes, and T cells in equal amounts. The cDNA subtractions were performed in either direction. Subtracted DC cDNA clones were immobilized on a microarray and subjected to differential screening. Among the clones selected for DC-preferred transcription, redundant clones were screened out and the remainder were further investigated for sequence identity. For the ones of interest, a DC subset–specific expression profile was elucidated by a newly designed microarray and RT-PCR analysis.