Expression profile of DCassociated genes in each DC subset.

Semiquantitative RT-PCR was performed on cDNA isolated from MoDCs, CD1a⁺ DCs, CD11c⁻ DCs, and CD14⁺DCs, B cells, monocytes, and T cells, respectively, and the PCR products were analyzed at 25 and 30 cycles of PCR. The result of RT-PCR was summarized with differential marking: (+++) for higher expression and (++) for lower expression detectable after 25 cycles of PCR; (+) and (+/−) for expression detectable only after 30 cycles of PCR and marginally detectable even after 30 cycles of PCR, respectively. GAPDH was used for normalization of each cDNA amount. CD19, CD14, and CD28 were used as control genes for B cells, monocytes, and T cells, respectively. The ratio of DC/BMT represents the degree of DC specificity of each clone as determined by microarray analysis and as described in Table 1. Genes not detected by differential screening were indicated by an asterisk. ND denotes not determined in this study.