Human IL-17E transgenic construct and protein expression in mice.

(A) Transgenic IL-17E overexpression construct, ApoE–hIL-17E, indicating ApoE promoter and HCR upstream of subcloned hIL-17E cDNA sequence. Restriction sites used are indicated. (B) Mouse sera (5 transgenics, T1-T5, and 3 littermate controls, C1-C3) were run in reducing conditions on a 14% Tris-Glycine gel, probed with conditioned media containing anti–hIL-17E, followed by incubation with HRP-linked secondary antibody (1:4000) and detected by chemiluminescence; 10-second exposure.