Distinct granular populations arise in the hIL-17E transgenic bone marrow.

(A) FACS light scatter dot plots of nontransgenic control (left plot) and transgenic (right plot) bone marrow cells, identifying novel highly granular, increased population in the transgenic animal. This population is indicated in pink, correlating to a 30% increase over the control. Plots represent one transgenic animal (Tg, n = 5; all displayed similar phenotype). (B) Sort of the transgenic granulocytic population reveals immature eosinophils and neutrophils comprise this population. Bone marrow from one high-expressing hIL-17E transgenic line was sorted based on high scatter properties identified in panel A and cytospots of sorted cells were stained with either CR (upper panel) or MPO (lower panel). Inset of CR staining represents transgenic bone marrow sort using CD11c-FITC and CD45-PE marker as well as light scatter plots.