Unique multimeric structure of patient's plasma VWF.

Plasma samples were obtained from the patient and a family member (mother and daughter, respectively) and 5 VWD controls, analyzed by means of agarose gel electrophoresis (1% agarose), and detected by Western blot with AVW-5 and AVW-17. Control plasma samples included normal pool plasma (NP, lane 3); thrombotic thrombocytopenic purpura (TTP, lane 4); and type 2A (lane 5), type 2B (lane 6), type 1 (lane 7), and type 3 (lane 8) for typing comparisons. Plasma samples obtained from the mother and daughter are shown in lanes 1 and 2. The VWF multimeric patterns of both patients (lanes 1 and 2) do not resemble any of the known type 2 VWD multimeric patterns.