Survival and proliferation of CFSE-labeled DLI cells.

Survival and proliferation of CFSE-labeled DLI cells in a week 3 DLI (A,C,E) and a week 12 DLI chimera (B,D,F), 14 days after DLI with CFSE-labeled cells. (A,B) Distribution of PBLs and the CD3⁺population according to CFSE fluorescence in a week 3 DLI (A) and a week 12 DLI chimera (B). (C,D) Histograms represent the CD3⁺ population only, obtained by gating in the corresponding dot plots, shown immediately above (CD3⁺ gate in panels A and B, respectively). Open histograms show the distribution of CD3⁺ cells according to CFSE fluorescence in the week 3 DLI (C) and the week 12 DLI chimera (D); M1 to M4 indicate cell populations with 2-fold decreasing CFSE fluorescence intensity; superimposed solid histograms represent spontaneous fluorescence of CD3⁺ cells from an age-matched control animal (given DLI with unlabeled cells). (E,F) Dot plots were obtained in the TCR-Vβ6⁺ CD3⁺ gate, identified, and gated using double labeling (not shown). Plots show TCR-Vβ6⁺CD3⁺ cells according to their CFSE fluorescence in the week 3 DLI (E) and the week 12 DLI chimera (F). Dot plots and histograms are shown from 1 of 5 animals within each group (1 of 2 representative experiments).