Expansion of splenic TCR-Vβ6⁺ T cells in GVHD.

The splenic frequency of T CD4⁺ and CD8⁺ T cells, expressing specific TCR-Vβ chains was determined by flow cytometry using FITC-labeled anti–Vβ-TCR monoclonal antibody and anti–CD4-PE and anti–CD8-PerCP monoclonal antibodies. Results are presented as percentage of total CD4⁺ (solid bars) and CD8⁺ (empty bars) T cells in animals, suffering from GVHD (GVHD; n = 2), in healthy control BM recipients (co; n = 2), and in untreated donor (C3H)-type and host (AKR)-type mice. TCR-Vβ11⁺ and TCR-Vβ8.3⁺ T-cell frequencies were determined as controls. Results are shown from 1 of 3 representative, identically designed experiments.