Host-reactive TCR-Vβ6⁺ T cells in PBLs.

Frequency of host-reactive TCR-Vβ6⁺ T cells in PBLs before and after DLI at 3 (upper panels) or 12 weeks (lower panels). Vβ6⁺ CD4⁺ (left panels) and Vβ6⁺ CD8⁺ (right panels) T cells were determined in PBLs by flow cytometry, using anti–TCR-Vβ6-FITC monoclonal antibody and anti–CD4-PE or anti–CD8-PE monoclonal antibody. Venous blood was taken from chimeras prior to and 3, 7, and 12 weeks after DLI at 3 or 12 weeks. Data represent mean ± SE of 2 (3 weeks) and 3 (12 weeks) identically designed experiments (n = 4-5/group and total n = 9 for 3 weeks, 14 for 12 weeks). *P < .05 for comparison between groups as tested by Kruskal-Wallis multiple comparison Z test.