Semiquantitative RT-PCR for IAPs in UCB CD34⁺ cells before and after growth factor stimulation.

Total RNA from CD34⁺ cells before (−) and after (+) Tpo, SCF, and FL stimulation was subjected to 30, 35, and 40 cycles of RT-PCR for all 7 known human IAPs and 25, 30, and 35 cycles for GAPDH. The number of PCR cycles is shown in parentheses. Total RNA derived from human melanoma G361 cells was used as positive control (PC). NC represents reactions without RNA template. Reaction products were visualized in 2% agarose gels stained with ethidium bromide.