Concentration kinetics of AAV/EGFP.

Primary CLL cells of 4 patients (17-20; Table 1) were infected at an MOI of 10, 100, or 500 with rAAV vectors encoding EGFP. The cells were analyzed for expression of EGFP by flow cytometry 48 hours after infection. Shown is the mean percentage of transduced cells with SEM (error bars). Infected cells (MOI of 500) were incubated with 500 IU heparin and EGFP expression detected 48 hours later.