Fig. 4.
Analysis of the ratio of ΔGT- to GTGT-containing sequence based on PCR product size.
32P-labeled PCR products encompassing the start of exon 2 from control individuals and carriers of the ΔGT mutation were separated on denaturing acrylamide sequencing gels. Bands were detected by autoradiography (as shown here) or in a Cyclone storage phosphor system. The upper (100 bp) and lower (98 bp) bands represent fragments containing the functional GTGT or the ΔGT mutation, respectively. Results from a single representative experiment are shown; mean values for each group are presented in Table 1.
