Fig. 5.
Fig. 5. In nonmalignant lymphocytes, the activity of μ-calpain is concentrated mostly in the T cells and decreases with age. / PBLs and immunomagnetically enriched CD3− and CD3−CD16− cells of a healthy young person were lysed in PBS and analyzed by casein zymography. Proteolytic bands corresponding to the activities of both isoenzymes were strongly seen only in the total peripheral blood lymphocyte (PBL) lysates, while the μ-calpain activity disappeared from the CD3−and CD3−CD16− cell lysates; std indicates the activities of standard μ- and m-calpain as in Figure 1 ([A] A representative zymogram of 1 of 3 similar experiments). Comparison of the activities of μ-calpain in the PBLs of 2 young, 2 middle-aged, and 2 old individuals shows a strong, age-related decrease of this activity ([B] Representative zymogram shows results of 1 of 3 similar experiments.

In nonmalignant lymphocytes, the activity of μ-calpain is concentrated mostly in the T cells and decreases with age.

PBLs and immunomagnetically enriched CD3− and CD3−CD16− cells of a healthy young person were lysed in PBS and analyzed by casein zymography. Proteolytic bands corresponding to the activities of both isoenzymes were strongly seen only in the total peripheral blood lymphocyte (PBL) lysates, while the μ-calpain activity disappeared from the CD3−and CD3−CD16− cell lysates; std indicates the activities of standard μ- and m-calpain as in Figure 1 ([A] A representative zymogram of 1 of 3 similar experiments). Comparison of the activities of μ-calpain in the PBLs of 2 young, 2 middle-aged, and 2 old individuals shows a strong, age-related decrease of this activity ([B] Representative zymogram shows results of 1 of 3 similar experiments.

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