Specificity of TRAIL-induced CD14 expression in HL60 cells.

After 24 hours of the indicated treatments, apoptosis (A) was determined by PI staining, and percentages of cells expressing CD14 (B) were evaluated by flow cytometry. Sup. indicates supernatant derived from either untreated or TRAIL-treated HL60 cultures. Data represent the means ± SD of 3 to 5 separate experiments performed in duplicate. (C) Evaluation of the phagocytosis ability of untreated and TRAIL-treated HL60 cells determined by flow cytometry measure of fluorescent latex microbead incorporation. Percentage of fluorescent cells is indicated. Data representative of 4 separate experiments are shown.