Effect of pharmacologic inhibitors on TRAIL-mediated cytotoxicity and CD14 induction in HL60 cells.

Cultures were left untreated or were treated for 24 hours with TRAIL in the absence (vehicle) or presence of the following inhibitors: L-NAME, inhibitor of NOS; z-VAD-fmk, inhibitor of effector caspases; z-IETD-fmk, specific inhibitor of caspase 8; z-LEHD-fmk, specific inhibitor of caspase 9. (A) Percentage of apoptosis quantitatively evaluated by flow cytometry after PI staining. (B) Percentage of cells expressing surface CD14, evaluated by flow cytometry. Data represent the means ± SD of 3 separate experiments performed in duplicate.