Fig. 1.
Platelet adhesion to decorin.
Polystyrene dishes were coated with 100 μg collagen (Coll), purified decorin (DCN), recombinant decorin (rDCN), or decorin protein core (DCN core). Control dishes were coated with BSA. Gel-filtered platelets (1 × 108 platelets) were added to the dishes and incubated for 90 minutes at room temperature. Nonadherent cells were removed, and the percentage of adherent platelets was determined by a colorimetric assay as described in “Materials and methods.” Results are the means ± SD of 3 to 8 experiments.