Fig. 6.
Fig. 6. Effect of the preincubation of platelets with antibodies against different membrane receptors on adhesion to decorin. / Samples of gel-filtered platelets (1 × 108 platelets, 0.5 mL) were incubated with buffer (none), 1 μg unrelated mouse IgG (control IgG), 1 μL anti-GPIb ascitic fluid AK2, 1 μg anti-CD38 monoclonal antibody IB4, 1 μg anti-CD31 monoclonal antibody Moon-1, 1 μg anti-integrin αIIbβ3 monoclonal antibody P2, 1 μg anti-integrin α2β1monoclonal antibody 1998, 1 μL anti-integrin α2β1 ascitic fluid P1E6, or 1 μL anti-integrin α2β1 ascitic fluid P1E6 pretreated with protein G-Sepharose for IgG depletion (IgG-depleted P1E6). After 5 minutes of incubation, platelet samples were added to decorin-coated dishes and allowed to adhere for 90 minutes. Results are the mean ± SD of 3 to 4 separate experiments and are expressed as percentage of platelet adhesion, considering 100% the adhesion measured in the absence of added antibodies.

Effect of the preincubation of platelets with antibodies against different membrane receptors on adhesion to decorin.

Samples of gel-filtered platelets (1 × 108 platelets, 0.5 mL) were incubated with buffer (none), 1 μg unrelated mouse IgG (control IgG), 1 μL anti-GPIb ascitic fluid AK2, 1 μg anti-CD38 monoclonal antibody IB4, 1 μg anti-CD31 monoclonal antibody Moon-1, 1 μg anti-integrin αIIbβ3 monoclonal antibody P2, 1 μg anti-integrin α2β1monoclonal antibody 1998, 1 μL anti-integrin α2β1 ascitic fluid P1E6, or 1 μL anti-integrin α2β1 ascitic fluid P1E6 pretreated with protein G-Sepharose for IgG depletion (IgG-depleted P1E6). After 5 minutes of incubation, platelet samples were added to decorin-coated dishes and allowed to adhere for 90 minutes. Results are the mean ± SD of 3 to 4 separate experiments and are expressed as percentage of platelet adhesion, considering 100% the adhesion measured in the absence of added antibodies.

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