Effect of TNF-α and LY294002 on AnV and propidium iodide staining in human neutrophils.

Human neutrophils were incubated in the absence (A) or presence (B) of TNF-α (200 U/mL) or LY294002 (10 μM; C) for 20 hours. Cells were harvested and stained with AnV (a marker of PS exposure/apoptosis) and PI (a marker of plasma membrane integrity/postapoptotic necrosis), as detailed in “Materials and methods.” TNF-α and LY294002, while not influencing the ratio of AnV high to low staining, increased the proportion of PI-positive cells within the AnV high pool. Representative data are shown from a single experiment with identical results obtained in 7 independent experiments.