Mutant forms of α-synuclein (A30P, A53T, A30P/A53T), β-synuclein, and α-synuclein112 function like the wild-type α-synuclein.

(A) α-Synuclein point mutants (A30P, A53T), found in the early-onset familial PD patients, and a double mutant (A30P/A53T) inhibits the ionomycin-induced PF4 release in a similar way to that found in wild-type. Below the figure, each protein for the quantitative comparison was loaded in SDS-PAGE and stained by Coomassie brilliant blue R-250. (B) β-Synuclein and (C) α-synuclein112 also inhibit the PF4 release. The PF4 released was measured as in Figure 1.