Intracellular Ca⁺⁺ level and increase of intracellular Ca⁺⁺ level on stimulation in the presence and absence of α-synuclein.

Purified platelets were incubated with Fura-2/am for 1 hour at room temperature and were washed with Tyrode solution. Fura-2/am fluorescence was then measured using a fluorescence spectrophotometer. (A) At t = 60, after the start of the run, 10 μM α-synuclein was added. (B) At t = 60, after the start of the run, 0.5 μM ionomycin was added in the presence or absence of 10 μM α-synuclein.