Morphologic changes of platelets on stimulation in the presence and absence of α-synuclein in electron microscopy.

Purified platelets were prepared as described in “Materials and methods” and were fixed for TEM analysis. (A, B) Platelets in the resting state in the absence (A) and presence (B) of recombinant α-synuclein. (C) Platelets that have been stimulated with 0.5 μM ionomycin for 5 minutes before fixation. (D) Platelets that were preincubated with 10 μM recombinant α-synuclein before stimulation with 0.5 μM ionomycin. Scale bars, 1μm.