A mini-EBV plasmid for immortalization of B cells and expression of pp65.

(A) Map of the pp65 mini-EBV plasmid. The pp65 gene is constitutively expressed from an SV40 promoter. (B) Expression of pp65 in mini-EBV–immortalized B-cell lines (mini-LCLs). A pp65 mini-LCL (solid line), a control mini-LCL (thin line), and an EBV B95.8 LCL (dotted line) from the same donor were fixed, permeabilized, stained with monoclonal pp65 antibody 981, and analyzed on a flow cytometer. Upper plot, cell lines from donor a41; lower plot, cell lines from donor F22. (C) Surface expression of MHC class I and II, B7.1 and 2, LFA-1, LFA-3, and ICAM-1 on a pp65 mini-LCL, a control mini-LCL, and a B95.8 LCL from donor no. 4.