Characterization of pp65 mini-LCL–stimulated T cells from 3 randomly selected healthy donors.

(A) Cytotoxic activity of pp65 mini-LCL–stimulated PBMC cultures from donors F14, F16, and F22 against autologous pp65 mini-LCLs (♦) or control mini-LCLs (■) as determined in a TDA release assay on day 17 of culture. (B) Proliferation of these cultures. (C) Flow cytometry analysis of the expression of CD4 and CD8 in cultures from donors F14 and F22 on day 33 of culture. (D) Cytotoxic activity of pp65 mini-LCL–stimulated cultures from donors F14 and F22 against autologous or allogeneic pp65 mini-LCLs (♦) or control mini-LCLs (■). Chromium release assays were performed on day 42 (F14 effectors) or on day 62 (F22 effectors) of culture.