Properties of pp65 mini-LCL–stimulated and control mini-LCL–stimulated T cells from the CMV⁻, EBV⁺ donor no. 1.

(A) The cytotoxic activity of pp65 mini-LCL–stimulated polyclonal T cells (upper row) and control mini-LCL–stimulated T cells (lower row) from donor no. 1 against diverse target cells was assayed by chromium release on days 24, 42, and 55, respectively. (B) Tetramer analysis of the T-cell cultures using a pp65 epitope tetramer (NLV) and an EBV epitope tetramer (CLG), both HLA-A2-restricted, performed on day 35 of culture. E/T indicates effector-target ratio.