Analysis of pp65 mini-LCL–stimulated and control mini-LCL–stimulated T cells from the CMV⁺, EBV⁻ donor no. 2.

(A) The cytotoxic activity of pp65-mini-LCL–stimulated polyclonal T cells (upper row) and control mini-LCL–stimulated T cells (lower row) from donor no. 2 against diverse target cells was assayed by chromium release on days 27, 42, and 53, respectively. (B) Analysis of the 2 T-cell populations with CD4- and CD8-specific antibodies and NLV, IPS, and YPL tetramers, performed on day 52 of cultivation.